CDP-Star is a sensitive, chemiluminescent substrate for alkaline phosphatase that allows for the rapid, reproducible detection of alkaline phosphatase-labeled molecules in Northern, Southern, and Western blotting applications. Detection of alkaline phosphatase-labeled molecules with CDP-Star is extremely sensitive as a result of low background luminescence coupled with high intensity and prolonged light output from the enzyme catalysis. Following exposure to alkaline phosphatase-labeled molecules, maximum light emission occurs at approximately 60 minutes and continues for up to 24 hours, allowing for multiple film exposures and/or the sensitive detection of targets present in small amounts. For convenience, CDP-Star is supplied as a 0.25 mM, ready-to-use aqueous solution (i.e. no dilution is necessary). CDP-Star functions on both neutral and positively-charged nylon, giving the reagent added application versatility.

Chemiluminescent substrates provide highly sensitive replacements for the widely used fluorogenic substrate methylumbelliferyl phosphate (MUP), and the colorimetric substrate p-nitrophenyl phosphate (pNPP). Low background luminescence coupled with high-intensity light output enables detection of alkaline phosphatase labels with the highest possible sensitivity and signal-to-noise.

CDP-Star is a chemiluminescent substrate for detection of alkaline phosphatase conjugates in membrane applications. The substrate will immediately begin to emit light upon mixing with alkaline phosphatase with peak light emission occurring after 2-4 hours and continuing for several days. Applications include Northern, Southern, and Western blotting.

Concentrate ：0.25 mM in H2O ,0.4 mM in H2O ,12.5 mM in H2O, 25 mM in H2O

Size: 10ml, 50ml, 100ml , 250ml, 500ml, 1000ml